Saturation of Mylotarg to myeloid cells in vivo.

Saturation of Mylotarg just before and 3 and 6 hours after the start of each Mylotarg treatment cycle was analyzed by comparing Mylotarg bound in vivo with the maximal Mylotarg binding. The different myeloid cells were determined by their scatter and CD45-binding characteristics: AML blast cells (hatched bars; cycle 1: n = 86; cycle 2: n = 35), monocytes (gray bars; n = 33; n = 45), and granulocytes (white bars; n = 55; n = 32). Significant differences (P < .05; indicated by the asterisks) were observed between cycle 1 and cycle 2 at the 0-hour time point.