Modulation of Mylotarg by myeloid cells in vivo.

Modulation of Mylotarg was analyzed 3 and 6 hours after the start of each Mylotarg treatment cycle by comparing the maximal Mylotarg binding at each time point with the maximal Mylotarg binding just before the start of the corresponding treatment cycle. The different myeloid cells were analyzed separately, based on their scatter or CD45-binding characteristics: AML blast cells (hatched bars; cycle 1: n = 86; cycle 2: n = 34), monocytes (gray bars; n = 33; n = 45), and granulocytes (white bars; n = 54; n = 32).