Chemotactic response of cells to chemokines or W peptide.

Hos/CD4/CCR5 or Hos/CD4/CXCR4 cells were stably transfected with FPRL1 cDNA or pcDNA3 vector alone. Cell migration in response to chemokines was examined. (A) Migration of Hos/CD4/CCR5 cells in the presence (FPRL1⁺) or absence (Mock) of FPRL1 in response to MIP-1α or W peptide compared to spontaneous migration (medium). ▨ indicates FPRL1⁺; ▪, Mock. (B) Migration of Hos/CD4/CXCR4 cells in the presence (FPRL1⁺) or absence (Mock) of FPRL1 in response to SDF-1α or W peptide compared to migration in the absence of chemoattractants (medium). ■ indicates FPRL1⁺; ▪, Mock. *P < .05 and **P < .01.