Effect of W peptide on macrophage response to chemokines RANTES and SDF-1α.

Human macrophages generated by incubating monocytes with 5% human AB serum for 4 days were preincubated at 37°C with W peptide (1 μM, 1 hour; W pep) of staurosporine (1.4 ng/mL, 1 hour) followed by W peptide (1 μM, 1 hour; Stauro+W pep). The cells were washed and examined for Ca⁺⁺ flux (A) and chemotaxis (B) in response to RANTES or SDF-1α (50 ng/mL each). The asterisk indicates significantly reduced cell response in cells treated with W peptide in comparison with cells treated with medium (Medium).