Inhibition of HIV-1 Env-mediated fusion by W peptide.

Hos/CD4/CCR5 (▨ indicates FPRL1⁺; ░, Mock) (A) or Hos/CD4/CXCR4 (B) cells in the presence (FPRL1⁺) or absence (Mock) of FPRL1 were pretreated with different concentrations of W peptide at 37°C for 1 hour, then were mixed with HeLa cells expressing R5 HIV-1 Env or TF-228 cells expressing X4 HIV-1 Env. After 12 hours of incubation at 37°C, the cells were lysed, and the lysate was allowed to react with chlorophenol red-β-mercaptopyranoside (CPRG) followed by measurement of OD at 570 nm. ▨ indicates FPRL1⁺; ▪, Mock.