American Society of Hematology

Fig. 1.

$Fig. 1. SDS-PAGE and size-exclusion chromatography of plasma-derived and recombinant proteins. / (A) SDS-polyacrylamide gel of proteins stained with Gelcode Blue. All lanes contain unreduced protein except for lane 4. Lane 1, human plasma FXI; lane 2, human recombinant FXI; lane 3, recombinant FXI-Ala321; lane 4, human plasma FXI (reduced); and lane 5, human plasma PK. (B) Retention times of proteins shown in panel A size-fractionated on a Superose-12 gel filtration column. The numbers next to the protein peaks correspond to the numbers used in panel A. The peak for human plasma FXI (lane 1) is drawn as a solid line and FXI-Ala321 (lane 3) as a dashed line. (C) Nonreducing SDS-polyacrylamide gel of chimeric proteins stained with Gelcode Blue. Lane 1, human plasma FXI; lane 2, FXI/PKA4; lane 3, human plasma PK; and lane 4, FXI/PKA4-Gly326. (D) Retention times of proteins shown in panel C size-fractionated on a Superose-12 gel filtration column. The numbers next to the protein peaks correspond to numbers in panel C. For panels A and C, positions of molecular mass standards in kilodaltons are shown at the left of the figures. For panels B and D, retention times of molecular mass standards are shown at the top of the figures.$

SDS-PAGE and size-exclusion chromatography of plasma-derived and recombinant proteins.

(A) SDS-polyacrylamide gel of proteins stained with Gelcode Blue. All lanes contain unreduced protein except for lane 4. Lane 1, human plasma FXI; lane 2, human recombinant FXI; lane 3, recombinant FXI-Ala321; lane 4, human plasma FXI (reduced); and lane 5, human plasma PK. (B) Retention times of proteins shown in panel A size-fractionated on a Superose-12 gel filtration column. The numbers next to the protein peaks correspond to the numbers used in panel A. The peak for human plasma FXI (lane 1) is drawn as a solid line and FXI-Ala321 (lane 3) as a dashed line. (C) Nonreducing SDS-polyacrylamide gel of chimeric proteins stained with Gelcode Blue. Lane 1, human plasma FXI; lane 2, FXI/PKA4; lane 3, human plasma PK; and lane 4, FXI/PKA4-Gly326. (D) Retention times of proteins shown in panel C size-fractionated on a Superose-12 gel filtration column. The numbers next to the protein peaks correspond to numbers in panel C. For panels A and C, positions of molecular mass standards in kilodaltons are shown at the left of the figures. For panels B and D, retention times of molecular mass standards are shown at the top of the figures.

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