Fig. 1.
Isolation of subpopulations of CD34+ BM cells on the basis of the relative intensity of Hoechst 33342 and Rhodamine 123 fluorescence.
After gating on live cells, the CD34+HoLow/RhoLow, CD34+HoLow/RhoInt, and CD34+HoLow/RhoHi subpopulations were isolated flow-cytometrically.