BrdU fluorescence intensity of granulocytes following withdrawal of BrdU feeding (PA6588).

(A) (B) (C) The BrdU fluorescence of BM granulocytes from the animal at different time points following withdrawal of BrdU (Day-0, panel A; Day-20 panel B; and Day 159, panel C) was analyzed flow-cytometrically. The MFI was determined by dividing the geometric mean fluorescence of BrdU-labeled granulocytes by the geometric mean fluorescence of control (BrdU nonexposed) granulocytes. Dotted green line histograms represent the MFI of granulocytes from BM of an animal not exposed to BrdU; continuous red line histograms indicate the MFI of granulocytes from the BrdU-fed animal following the withdrawal of BrdU. (D) (E) (F) (G) (H) The MFI of KG1a cells exposed to BrdU for 9 hours and cultured for an additional 4 days in the absence of BrdU. Dotted green line histograms represent the MFI of KG1a cells not pulsed with BrdU (BrdU⁻), and continuous red line histograms represents the MFI of BrdU-pulsed KG1a cells at different time points. The relative decrease of MFI of KG1a cells was used to determine the number of cell divisions that KG1a cells underwent during this time period; this was done by analyzing the decrease in MFI.