Relative IL-10 and GAPDH mRNA content in patient tissues.

Shown are autoradiographs of gels containing radiolabeled IL-10 (upper panel) and GAPDH (lower panel) cDNAs prepared by RT-PCR on tissue extracts. Lane 1 shows IL-10 cDNA and GAPDH cDNA in IL-10–producing positive control cells. Lane 2 shows the result of an RT-PCR reaction on water without added cell RNA extracts. Lanes 3 to 7 show RT-PCR products from tissues of 5 ALPS patients. Lanes 8 and 9 show products of reactions with RNAs from tissues showing reactive lymphoid hyperplasia (RLH). The relative GAPDH signals (lower panel) are similar for all specimens, but the IL-10 signals (upper panel) are markedly more intense for ALPS specimens.