Fig. 2.
Role of integrins in PI3K-mediated increases in PMN transmatrix migration.
Isolated human PMNs were pre-exposed to indicated concentrations of wortmannin and/or antibodies (10 μg/mL for each) directed against MHC class I (▪), anti–β1-integrin (▧), anti–β2-integrin (░), or anti–β3-integrin (▤) followed by examination of fMLP-stimulated (10−8 M) transmigration across matrix-coated substrates (A). Transmigrated PMNs were quantified by determination of MPO. Panel B represents data converted to percent inhibition relative to control binding antibody. Data are derived from 9 to 12 monolayers in each condition from at least 3 separate experiments with results expressed as mean ± SEM number transmigrating PMNs .
