Fig. 3.
PI3K-mediated uncoupling of β3-integrin–mediated inhibition of PMN transmatrix migration; antibody dose response.
Isolated human PMNs were pre-exposed to wortmannin (100 nM, ■) or vehicle (▪) and/or indicated concentrations of antibody directed against β3-integrin followed by examination of fMLP-stimulated (10−8 M) transmigration across matrix-coated substrates (A). Transmigrated PMNs were quantified by determination of MPO content following termination of the assay. Panel B represents data converted to percent inhibition relative to no antibody controls. Data are derived from 8 to 10 monolayers in each condition from at least 3 separate experiments with results expressed as mean ± SEM number transmigrating PMNs .
