Fig. 4.
Inhibition of PI3K blocks fMLP-activated β3-integrin tyrosine phosphorylation.
Isolated human PMNs were pre-exposed to vehicle or wortmannin (3 nM in the top panel or indicated concentration in the bottom panel) followed by adhesion to matrix substrates. Adherent PMNs were exposed to fMLP (10 nM) for indicated periods of time (top panel) or for 10 minutes (bottom panel). Cells were lysed and β3-integrin was immunoprecipitated and resolved by SDS-PAGE. Tyrosine phosphorylation was assessed by immunoblot. Data are representative from 3 separate experiments.
