Monocyte TF in RNA synthesis.

(A) Tissue factor mRNA synthesis in immunopurified monocytes after the incubation of normal (C1-C2) or HIT IgG (P1-P5) in whole blood at 500 μg/mL in the absence (φ) or the presence of PF4 (10 μg/mL) with heparin (0.25 IU/mL) (H/PF4). Whole blood was also incubated in the presence of H-PF4 complexes without IgG or in the presence of LPS as a positive stimulation control (LPS). (B) Effects of IgG purified from one sample of normal plasma (C1) and from 2 selected patients (P2 and P3) tested alone (φ) or in the presence of PF4 without (PF4) or with (H/PF4) heparin.