American Society of Hematology

Fig. 3.

$Fig. 3. Separation of desialylated oligosaccharides of L8D and 6-19J IgG3 mAb by ODS-HPLC. / Desialylated ABEE-oligosaccharides of L8D (A) and 6-19J (B) IgG3 were separated into 8 oligosaccharide fractions that were eluted as a series of authentic bi-antennary complex-type oligosaccharide structures (positions 1 to 8) of ±Galβ1-4GlcNAcβ1-2Manα1-6(± Galβ1-4GlcNAcβ1-2Manα1-3)Manβ1-4GlcNAcβ1-4(± Fucα1-6)GlcNAc shown in the right column. Peaks 1 and 5 correspond to digalactosylated oligosaccharide chains with or without a fucose residue (G2). Peaks 2, 3, 6, and 7 correspond to monogalactosylated oligosaccharide chains with or without a fucose residue (G1). Peaks 4 and 8 correspond to nongalactosylated oligosaccharide chains with or without a fucose residue (G0). G and Gal, galactose; GN and GlcNAc,N-acetylglucosamine; M and Man, mannose; F and Fuc, fucose.$

Separation of desialylated oligosaccharides of L8D and 6-19J IgG3 mAb by ODS-HPLC.

Desialylated ABEE-oligosaccharides of L8D (A) and 6-19J (B) IgG3 were separated into 8 oligosaccharide fractions that were eluted as a series of authentic bi-antennary complex-type oligosaccharide structures (positions 1 to 8) of ±Galβ1-4GlcNAcβ1-2Manα1-6(± Galβ1-4GlcNAcβ1-2Manα1-3)Manβ1-4GlcNAcβ1-4(± Fucα1-6)GlcNAc shown in the right column. Peaks 1 and 5 correspond to digalactosylated oligosaccharide chains with or without a fucose residue (G2). Peaks 2, 3, 6, and 7 correspond to monogalactosylated oligosaccharide chains with or without a fucose residue (G1). Peaks 4 and 8 correspond to nongalactosylated oligosaccharide chains with or without a fucose residue (G0). G and Gal, galactose; GN and GlcNAc,N-acetylglucosamine; M and Man, mannose; F and Fuc, fucose.

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