Fig. 4.
Binding of MBL to L8D and 6-19J IgG3 mAb.
Five micrograms purified mAb were adsorbed on a polyvinylidene difluoride membrane heated at 100°C for 10 minutes, incubated with125I-labeled MBL, and subjected to autoradiography for 24 hours for the detection of MBL binding.