Fig. 3.
Effect of binding to α2M or α2M* on FGF-2–induced endothelial cell proliferation.
FGF-2, 0.5 mg/mL, was incubated with the indicated concentrations of α2M or α2M* for 2 hours at 37°C. This mixture was then added to quiescent FBHE cells, and cell proliferation was determined by [methyl-3H]thymidine incorporation as described in “Materials and methods.” FGF-2 activity in the absence of α2M or α2M* was defined as 100%. Data presented are the mean ± SD from 4 experiments.