MoAb 97A6 recognizes E-NPP3.

HEK 293 cells were transfected with full-length E-NPP3 cDNA and the resulting cells were screened for reactivity with MoAb 97A6. The positive population was sorted by FACS and cultured in RPMI 1640 plus 10% fetal bovine serum. After 3 rounds of sorting, the cells were stained either with control IgG1-PE (dotted histogram) or 97A6-PE (filled histogram) and analyzed on a FACSCalibur flow cytometer. Nontransfected 293 cells were negative for MoAb 97A6 (not shown).