Effect of BB3103 and BB94 on bFGF/TNF-α–induced tube formation and u-PA accumulation.
The hMVECs were cultured on top of a 3-dimensional fibrin matrix in M199 supplemented with 10% human serum and 10% NBCS (control), or with bFGF (10 ng/mL) and TNF-α (10 ng/mL) in the presence of increasing amounts of BB3103 or 5 μg/mL BB94. (A) After 7 days of culture, total tube length (mm/cm2) was measured using image analysis equipment as described in “Materials and methods.” The data are expressed as the mean of duplicate wells, with the range of the 2 measurements indicated by the error bars, and are representative of 3 independent experiments, respectively. (B) Accumulation of u-PA in the supernatants of the stimulated hMVECs was measured by ELISA as described. The data of control (■), bFGF and TNF-α (▪), and bFGF/TNF-α–stimulated hMVECs in the presence of 25 μg/mL BB3103 (▵) or 5 μg/mL BB94 (▿) are expressed as the mean of duplicate wells (nanograms per well), with the range of the 2 measurements indicated by the error bars, and are representative of 3 independent experiments.
