Expression of u-PAR mRNA in hMVECs after BB94 treatment.

Confluent hMVECs cultured on gelatin-coated wells were preincubated without (control) or with bFGF (10 ng/mL) and TNF-α (10 ng/mL) in the presence of vehicle (0.1% DMSO) or 10 μg/mL BB94 and 0.1% DMSO for the indicated period. Then the hMVECs were restimulated, and total RNA was isolated at 8 hours and analyzed by Northern blotting for u-PAR mRNA (A). Equal loading was checked by hybridization with an actin probe. Signals for u-PAR were quantified by densimetry and adjusted for the corresponding actin signal (B). The amount of u-PAR mRNA present at the different times is expressed relative to that found under control conditions. Two independent experiments gave similar results.