Fig. 8.
![Fig. 8. Inhibition of the cleavage of domain D1 from domain D1D2D3 of u-PAR by BB94 in the supernatants and on the cell surface of hMVECs. / (A) The hMVECs were stimulated with or without 10−8 M phorbol ester PMA in the absence or presence of 10 μg/mL BB94 for 24, 48, or 72 hours or (B) the combination of 10 ng/mL bFGF and 10 ng/mL TNF-α in the absence or presence of 10 μg/mL BB94 for 72 hours. Supernatants were collected, and Triton X-100 cell lysates were prepared (only after 72 hours [C]). Immunoprecipitation and Western blot analysis were performed as described in “Materials and methods.” CM indicates conditioned medium.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/10/10.1182_blood.v97.10.3123/6/h81011057008.jpeg?Expires=1735610636&Signature=t-7smhXnltquzdt6ZLSEIYqT6v76yz8aq3sCHGb0qI5COXvgtOArhphUW5ZurtRJD6eMXP~jm9QEIX3n6O0RkBfg0zh4I4kGtHHEcJwxPIjHzMf1F2HSYTby-HBAYFUMABT5ycVWQ~wTffaiKo-e~9isRIcIGOTe73OyvT0VIGS4YPAsodQHyIuS1sNJ0r-VrhM0Ger~aEZP26mgmX8ogPg5dtdbNJr1gMrwcxtyi0Weg3JJbA0htoINhuNAVmp3Q0EiVFTLz1Xk9eMBx4rgNSmxXOKxbMZMCWLOEbyZQWO6iQJcYOYG7CSRYqhHxB1WaJ4BGM~pAtpOWYQN15RioA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Inhibition of the cleavage of domain D1 from domain D1D2D3 of u-PAR by BB94 in the supernatants and on the cell surface of hMVECs.
(A) The hMVECs were stimulated with or without 10−8 M phorbol ester PMA in the absence or presence of 10 μg/mL BB94 for 24, 48, or 72 hours or (B) the combination of 10 ng/mL bFGF and 10 ng/mL TNF-α in the absence or presence of 10 μg/mL BB94 for 72 hours. Supernatants were collected, and Triton X-100 cell lysates were prepared (only after 72 hours [C]). Immunoprecipitation and Western blot analysis were performed as described in “Materials and methods.” CM indicates conditioned medium.
