Effect of AdSDF1 on peripheral blood cell counts.
(A) Some SCID mice were treated with 1 × 109 pfu AdSDF1 or AdNull IV on day 0. Total white blood cells (○, AdSDF1; ●, AdNull) and granulocytes (■, AdSDF1; ▪, AdNull) were counted using a Neubauer hematocytometer on crystal violet stained. (B) Some SCID mice were treated with 1 × 109 pfu AdMIP3α or AdNull IV on day 0. Total white blood cells (○, AdMIP3α; ●, AdNull) and granulocytes (■, AdMIP3α; ▪, AdNull). All data are expressed as mean (n = 4 to 6) ± SEM. (C) Effect of AdSDF1 on differential leukocyte counts. Two strains of mice were treated with 1 × 109 pfu AdNull or AdSDF1 or AdMIP3α IV on day 0. Manual leukocyte differentials were performed on Wright-Giemsa–stained smears of peripheral blood. All data are expressed as mean percentages (n = 4 to 6). (D) Phenotypic characterization of peripheral blood mononuclear cells. Peripheral blood MNCs were prepared from AdSDF1-treated or AdNull-treated SCID mice on day 5 and stained with FITC-conjugated anti-CD11b and PE-conjugated anti–Gr-1, or FITC-conjugated anti-CD34 and PE-conjugated anti–Sca-1 MoAbs. Cells (1 × 104) were analyzed on a Coulter Elite flow cytometer. The representative percentages of positive populations in PBMNCs are shown.
