The effects of transfusion on patient flow cytometry results.

Dot plots for the dual staining of red blood cell spheres with Helix and Ulex are shown for an A1 patient before transfusion (A) and after an A2 blood transfusion (B). The A2 population is located within quadrant 1 and comprises 18% of the red blood cell population. An A2 blood group patient before and after transfusion with A1 blood is also shown (C and D) with the A1 population comprising 16% of the red blood cell population after transfusion. The LFL1 axis shows Ulex fluorescence and the LFL2 axis shows Helix (A) fluorescence.