sTNFRI/Fc chimera blocks the sensitization of CFU-C from NK cell–deficientRAG2-γc−/−spleens to Fas-induced apoptosis on the addition of sorted NK cells.
(A) Total splenocytes (106 cells/mL) from NK cell–deficient mice were incubated for 24 hours in culture medium or anti-Fas mAb (5 μg/mL) plus IFN-γ (100 U/mL) with or without sorted NK1.1+ cells (2 × 105 cells /mL) from wild-type mice. Cells were then recovered and evaluated for colony growth in methylcellulose. Data are means ± SEM from triplicate determinations and represent the typical results of 1 of 2 experiments. (B) The same experiment was performed with sorted B220+ and B220− NK cell subsets with or without sTNFRI/Fc chimera (10 ng/mL). Data are means ± SEM from triplicate determinations. NS, not significant in comparison with controls.
