Fig. 5.
Up-regulation of Fas expression on progenitor-enriched Lin− spleen cells.
Splenocytes were incubated overnight (15 hours) in culture medium alone (broken line) or together with IFN-γ (100 U/mL) (A), TNF-α (10 ng/mL) (B), or both (solid line) (C). Cells bearing lineage markers were labeled with a cocktail of 5-fold diluted hybridoma supernatants (B220, Thy1, TER119, and GR-1) revealed by mouse antirat F(ab')2. After staining with PE-conjugated anti-Fas mAb (Jo2) (broken and solid line) or isotype control (dotted line), Fas expression was analyzed on gated Lin− cells.
