Immunoprecipitation of SP-IR in the sera of subjects with and without BM fibrosis.

Sera (1 mL) were immunoprecipitated with anti-SP and then analyzed by Western blot for SP with the same antibody. (A) Representative samples are shown for patients with fibrosis: IMF, ET/IMF, and CML. Lanes are shown for results obtained for NC or sera with fewer than 5 pg/mL SP and no fibrosis. (B) Immunoprecipitates were analyzed with anti-SP pre-absorbed with SP. Lane 1, regular anti-SP; lane 2, pre-absorbed anti-SP.