Northern blot analysis of

c-cis expression in 32D cells with and without treatment with the SCFA derivatives. After the addition of 0.5 U/mL IL-3 at time zero, mRNA was prepared from the 32D cells at 0, 1, and 3 hours, as labeled above each lane. In the control lane (IL-3) 0.5 U/mL IL-3 was added at time 0, and in the EPO lane 3 U/mL was added at time 0 after starvation overnight. AB lanes had a concentration of 1 mM arginine butyrate; 1, 1 mM α methylhydrocinnamic acid; 2, 1 mM 2,2 dimethylbutyric acid; the cells were starved overnight in these concentrations and at time zero 0.5 U/mL IL-3 was added.