CD8⁺TCRαβ⁺ T cells promote complete donor-type chimerism and eliminate BCL₁ tumor cells in the peripheral blood 20 days after BMT.

(A) Two-color flow cytometric analysis of H-2K^b versus BCL₁-idiotype markers in white blood cells from a control recipient given BCL₁ and total body irradiation (TBI, 450 cGy) alone. (B) Analysis of a recipient given BCL₁ cells, TBI, and a transplant of 25 × 10⁶ wild-type C57BL/6 whole bone marrow cells. (C) An irradiated tumor-bearing recipient given 50 × 10⁶ CD4^−/− bone marrow cells. (D) Recipient given 50 × 10⁶ CD8^−/− bone marrow cells. (E) An irradiated tumor-bearing recipient given a transplant of 50 × 10⁶ TCD wild-type bone marrow cells. Add-back of 0.1 × 10⁶ sorted wild-type bone marrow CD8⁺ T cells to the transplant is shown in panel F. Percentage of cells in each quadrant is shown.