RT-PCR analysis of mRNA expression for various growth factors by CD34⁺ cells.

IGF-1 (A), FGF-2 (B), HGF (C), VEGF (D), and NGF-β (E). Lane 1, purified BM CD34⁺ cells; lane 2, BFU-E–derived cells; lane 3, CFU-GM–derived cells; lane 4, CFU-Meg–derived cells; lane 5, platelets; lane 6, stromal cells; lane 7, negative control of RT-PCR reaction (H₂O instead of mRNA). The data are representative of 4 to 8 experiments using cells separated from different donors.