American Society of Hematology

Fig. 3.

$Fig. 3. Concentration-dependent changes in mitochondrial membrane potential and levels of ROS after imexon treatment. / Reduction of the mitochondrial membrane potential (Δψm, A) and formation of reactive oxygen species (ROS, B) in RPMI 8226 myeloma cells treated with imexon for 48 hours as measured by CMXRos or HE staining and flow cytometry. Treatment with 200 μM tbhp for 30 minutes was used as positive control. Data are representative of 3 experiments. The fraction of cells staining with CMXRos having high Δψm and the fraction of cells staining with dihydroethidium (HE) corresponding to cells with increased levels of ROS are indicated.$

Concentration-dependent changes in mitochondrial membrane potential and levels of ROS after imexon treatment.

Reduction of the mitochondrial membrane potential (Δψ_m, A) and formation of reactive oxygen species (ROS, B) in RPMI 8226 myeloma cells treated with imexon for 48 hours as measured by CMXRos or HE staining and flow cytometry. Treatment with 200 μM tbhp for 30 minutes was used as positive control. Data are representative of 3 experiments. The fraction of cells staining with CMXRos having high Δψ_m and the fraction of cells staining with dihydroethidium (HE) corresponding to cells with increased levels of ROS are indicated.

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