Semiquantitative PCR of DNA fragments from untreated RPMI 8226 cells and cells treated with various imexon concentrations.

Panel A represents mitochondrial DNA fragments from control cells (0) and cells treated with 45, 90, and 180 μM imexon for 48 hours. AZQ was included as positive control (2.7 μM for 24 or 48 hours; lanes 5 and 6). Panel B represents nuclear DNA fragments from cells treated as above. The corresponding graphs represent relative densities of individual bands analyzed by the Eagle Eye II Video Still System (Stratagene).