Release of sCD100 from the surface of activated primary T and B cells.

Primary T and B cells were stimulated with ConA (4 μg/mL) or anti-CD40 mAb (HM40-3, 1 μg/mL), respectively, for 20 hours at 37°C. Cell lysates (1% NP-40) or culture supernatants were immunoprecipitated with BMA-12. Immunoprecipitates were subjected to SDS-PAGE in reducing conditions, and then electrophoretically transferred to nitrocellulose membranes and blotted with streptavidin-POD. Molecular weight markers (Mr) are indicated on the right.