Flow cytometric Vβ analysis in patients with mature T-cell proliferations compared with healthy controls.

Using Vβ8.1/8.2, Vβ13.1/13.3, and Vβ22 (FITC-labeled) double immunofluorescence stainings with CD3-PerCP, only small percentages of CD3⁺/Vβ⁺ cells can be identified in healthy controls (upper panel), whereas similar double stainings in samples 93-027, 93-067, and 96-049 enable identification of large T-cell populations with single Vβ8.1/8.2, Vβ13.1/13.3, and Vβ22 expression, respectively (lower panel).