Vβ-Cβ RT-PCR heteroduplex analysis.

After reverse transcription of total RNA of T-LGL patient 86-041, cDNA was PCR amplified using Vβ family primers in combination with a Cβ primer. On heteroduplex analysis, 2 major clonal products (Vβ1-Cβ and Vβ7-Cβ) belonging to the dominant clone were observed in addition to several weaker clonal Vβ-Cβ products. These latter products represented rearranged alleles of small subclones that were not identified with flow cytometric Vβ analysis.