American Society of Hematology

Fig. 1.

$Fig. 1. Morphology of in vitro–generated DCs from CLPs and CMPs. / (A) Typical clusters of DCs developing from 1 × 103 CLPs cultured with IL-1β, IL-3, IL-4, IL-7, SLF, TNF-α, and Flt3-L. Clusters were photographed directly in the Terasaki tray on an inverted microscope (objective × 40) on day 4 of culture. (B, C) Cells developing from 100 CLPs in the above cocktail on day 4 or from 5000 CMPs in IL-3, IL-4, SLF, TNF-α, Flt-3L, and GM-CSF on day 6 were dissociated with EDTA, spun onto slides, and stained with Giemsa (objective × 60 oil). Almost all CLP-derived cells (B) and a substantial fraction of CMP-derived cells (C) displayed typical DC morphologies varying in size and nuclear shape.$

Morphology of in vitro–generated DCs from CLPs and CMPs.

(A) Typical clusters of DCs developing from 1 × 10³ CLPs cultured with IL-1β, IL-3, IL-4, IL-7, SLF, TNF-α, and Flt3-L. Clusters were photographed directly in the Terasaki tray on an inverted microscope (objective × 40) on day 4 of culture. (B, C) Cells developing from 100 CLPs in the above cocktail on day 4 or from 5000 CMPs in IL-3, IL-4, SLF, TNF-α, Flt-3L, and GM-CSF on day 6 were dissociated with EDTA, spun onto slides, and stained with Giemsa (objective × 60 oil). Almost all CLP-derived cells (B) and a substantial fraction of CMP-derived cells (C) displayed typical DC morphologies varying in size and nuclear shape.

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