Phenotypic characterization of in vitro CLP- and CMP-derived DCs.

Cells were cultured as described in “Material and methods.” All cells were stained with anti–MHC class II and anti-CD11c (contour plot) plus 1 or 2 additional markers (histograms) by day 4 (CLP cultures) or day 6 (CMP cultures), respectively. Filled histograms show specific staining, and open histograms show isotype-matched controls of gated population in the contour plot (MHC class II⁺CD11c⁺). CLPs and CMPs do not express MHC class II (not shown).