Gene expression in CLPs and DCs.

RT-PCR analysis of CLPs, CLP-derived DCs in culture, CD8α⁺ and CD8α⁻ splenic DCs, total splenic cells, and CD8α⁺ thymic DCs sorted from healthy animals. Although CLPs do not express CIITA, ELC, andRelB, CLP-derived DCs in culture, splenic and thymic DCs express these genes. No DC subsets expressed CD3. Uncultured splenic and thymic DCs expressed CD8α⁺ mRNA. PCR conditions were as described in “Materials and methods.”