DCs derived from CLPs and CMPs were functionally active in allogeneic mixed leukocyte reactions.
(A) Stimulation of 105 allogeneic BALB/c lymph node cells by graded numbers (x-axis) of in vitro CLP-derived DCs (day 5 of culture) (closed diamonds) and by bone marrow (Lin−)-derived DCs (day 5 of culture) (open squares). Cultures were grown and MLR was performed as described in “Materials and methods.” Results are the means ± SE each with 3 to 6 wells per point. (B) MLR of in vivo CLP-, CMP-, and host-derived DCs sorted by high expression of CD11c and MHC class II. Sorted DCs were cultured in numbers as indicated for 12 hours in complete media without cytokines, and 2 × 105 nucleated BALB/c splenocytes were added. MLR was performed as described in “Materials and methods.” (A, B) Results of 3 representative experiments are shown.
