Fig. 2.
Fig. 2. SDS-PAGE analysis of 35S-methionine–labeled P2X1wt and P2X1del receptors after transcription and translation reactions. / Proteins translated from P2X1wt and P2X1delplasmid (pcDNA3.1) DNAs were radiolabeled with35S-methionine using a coupled transcription–translation (TnT) rabbit reticulocyte system in the presence or absence of microsomes. Note the differences in the apparent molecular sizes for translated proteins between different plasmid constructs, separated by SDS-PAGE, in the presence of microsomes (lanes 1 and 2) or in their absence (lanes 3 and 4). Note that each plasmid preparation contains only P2X1wt or P2X1del DNA and that, therefore, only homomeric receptor complexes can be formed.

SDS-PAGE analysis of 35S-methionine–labeled P2X1wt and P2X1del receptors after transcription and translation reactions.

Proteins translated from P2X1wt and P2X1delplasmid (pcDNA3.1) DNAs were radiolabeled with35S-methionine using a coupled transcription–translation (TnT) rabbit reticulocyte system in the presence or absence of microsomes. Note the differences in the apparent molecular sizes for translated proteins between different plasmid constructs, separated by SDS-PAGE, in the presence of microsomes (lanes 1 and 2) or in their absence (lanes 3 and 4). Note that each plasmid preparation contains only P2X1wt or P2X1del DNA and that, therefore, only homomeric receptor complexes can be formed.

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