Fig. 3.
Fig. 3. Immunoreactive characterization of the P2X1receptor. / Immunoprecipitation of surface-expressed P2X1wt and P2X1del receptors stably transfected into 1321 astrocytoma cells or on native megakaryoblastic or megakaryocytic cells. Surface-biotinylated 1321 cells transfected with P2X1wt(lanes 1 and 2) or 2 individual P2X1del receptors (DL 4 and DL 5, lanes 3-6), Dami cells (lanes 7 and 8), CMK 11-5 cells (lanes 9 and 10), or CMK cells (lane 11) were solubilized in RIPA buffer and antigens immunoprecipitated with the anti-hP2X1 pAb (+) but not with the preimmune antibody (−). Note that the apparent size of the single 67-kd protein is identical in direct comparisons between the P2X1wt and P2X1del clones and native Dami, CMK 11-5, and CMK cells.

Immunoreactive characterization of the P2X1receptor.

Immunoprecipitation of surface-expressed P2X1wt and P2X1del receptors stably transfected into 1321 astrocytoma cells or on native megakaryoblastic or megakaryocytic cells. Surface-biotinylated 1321 cells transfected with P2X1wt(lanes 1 and 2) or 2 individual P2X1del receptors (DL 4 and DL 5, lanes 3-6), Dami cells (lanes 7 and 8), CMK 11-5 cells (lanes 9 and 10), or CMK cells (lane 11) were solubilized in RIPA buffer and antigens immunoprecipitated with the anti-hP2X1 pAb (+) but not with the preimmune antibody (−). Note that the apparent size of the single 67-kd protein is identical in direct comparisons between the P2X1wt and P2X1del clones and native Dami, CMK 11-5, and CMK cells.

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