Flow cytometric representation of the phenotype of spleen cells 45 to 50 days after BMT.

BALB/c mice (H-2d) were submitted to a 6-Gy TBI and grafted 24 hours later with 10⁶ BM cells from FVB (H-2^q) mice alone or with apoptotic SCs. Between day 45 and day 50 post-BMT, engraftment was evaluated by FACS analysis. (A) This panel shows forward light scatter and side light scatter gating with 2 different regions: R1 (corresponding to lymphocytes that were confirmed by anti-CD3 mAb staining) and R2 (corresponding to monocytes and granulocytes, confirmed by anti-Gr1 and CD14 mAb staining). (B,D) These panels show H-2 expression on BALB/c (B, H-2^dPE⁺) and FVB (D, H-2^q FITC⁺) SCs. (C,E) These panels show a representative flow cytometry profile from an engrafted mouse that received 10⁶ FVB BM cells plus 5 × 10⁶ apoptotic irradiated FVB SCs. A donor phenotype (H-2^q) was observed in both lymphoid (C, R1 gating) and myeloid (E, R2 gating) lineages. The percentage of H-2^qdonor cells is indicated in each panel.