American Society of Hematology

Fig. 7.

$Fig. 7. Hemoglobin-oxygen dissociation curves. / Oxygenated hemolysates from β6I homozygotes (β6I/β6I), β6I heterozygotes (βWT/β6I), and wild-type mice (βWT/βWT), were deoxygenated incrementally with humidified O2-free nitrogen gas. O2fractional saturation of a hemolysate was determined by monitoring the change in absorbance at 555 nm versus O2 partial pressure. A fitted oxygen dissociation isotherm for each genotype (n = 3 for all groups) is plotted for derivation of a P50value.$

Hemoglobin-oxygen dissociation curves.

Oxygenated hemolysates from β^6I homozygotes (β^6I/β^6I), β^6I heterozygotes (β^WT/β^6I), and wild-type mice (β^WT/β^WT), were deoxygenated incrementally with humidified O₂-free nitrogen gas. O₂fractional saturation of a hemolysate was determined by monitoring the change in absorbance at 555 nm versus O₂ partial pressure. A fitted oxygen dissociation isotherm for each genotype (n = 3 for all groups) is plotted for derivation of a P₅₀value.

This Feature Is Available To Subscribers Only