Analysis of p53 protein expression in SLVL.

(A) Positive staining with anti-p53 antibody (clone DO7) using peroxidase-antiperoxidase method on a cytospin preparation of separated spleen cells from a patient with SLVL. p53 is visualized as brown precipitate in the nucleus of the cells. (B) Flow cytometric analysis of p53 protein expression in a patient with SLVL; cells were fixed with paraformaldehyde and permeabilized with 80% ethanol. In an indirect staining protocol, a monoclonal antibody (clone DO1) recognizing an epitope comprising amino acids 11 to 25 of the p53 molecule was used for detection of p53 expression or an isotypic control was used as first layer followed by a second layer—FITC-conjugated goat antimouse IgG F(ab)₂.