Semiquantitative assay of p16^INK4a and p14^ARF mRNA transcripts by RT-PCR in normal and PV ECFCs.

The sequence of primers is shown in Table 2. The length of the p16^INK4a amplification product is 142 bp and the length of p14^ARF is 212 bp. The marker sizes on the top panel are 750, 500, 300, 150, and 50 bp whereas the markers on the bottom panel are 1000, 750, 500, 300, and 150 bp. The amount of original total RNA used for the RT-PCR was 80 ng, and 20 ng, 5 ng and 1.25 ng, respectively, were used for the dilutions of 1:4 to 1:64.