Effects of anti–HLA class II mAb on antigen-induced proliferation of GPIIb-IIIa–reactive CD4⁺ T-cell lines.

GPIIb-IIIa–reactive T-cell lines were cultured with autologous antigen-presenting cells and IIIa22-262 for 3 days in the presence or absence of anti–HLA-DR, anti–HLA-DQ, anti–HLA-DP, or isotype-control mAbs, and ³H-thymidine incorporation was measured by liquid-scintillation counting. Anti–HLA class II mAb and isotype-control mAbs were added at the initiation of cultures. Only standard deviation > 20% of the mean and 100 cpm is shown as an extended line above a bar.