Fig. 1.
12B1 cells do not express Fas or respond to anti-Fas antibody.
(A) 12B1 cells do not express Fas on their surface. 12B1 cells were incubated with purified antimouse Fas–FITC-conjugated antibody, washed, and analyzed by flow cytometry. (B) Failure of increasing concentrations of antimouse Fas antibody to induce apoptosis in 12B1 cells in vitro as evaluated by MTT assay after 24 hours.