DCs pulsed with heat-stressed apoptotic cells induce a tumor-specific immune response.
(A) 12B1-D1 cells (5 × 105) were incubated for 6 hours with 40 nM AP20187 with or without an equal number of DCs, and 12B1-D1 cells were heat shocked for 1 hour at 42°C, then incubated with AP20187 and DCs for 6 hours. The cell mixtures were washed and injected SC into BALB/c mice. Mice were followed for tumor development (n = 8 mice per group). P < .01 for group 12B1-D1 + AP20187 versus 12B1-D1 + HS + AP20187 + DC from day 13 onward. (B) Naive and surviving BALB/c mice from (A) were rechallenged 40 days later with 2 × 104 12B1-D1 cells in the left groin (n = 5 surviving and 8 naive mice). (C) The same mice were challenged with 106 A20 cells in the right groin. Shown are representative data from a total of 3 experiments performed.
