Fig. 1.
Rapa does not affect phenotypic differentiation or CD40L-induced maturation.
Monocytes were cultured with IL-4 and GM-CSF in the absence (control) or presence of 10−7 M Rapa. On day 6, cells were harvested, extensively washed, and cocultured with L-Orient or L-CD40L for the subsequent 48 hours. On day 8, cells were harvested and analyzed for several surface markers by flow cytometry. Mean fluorescence intensity of DCs stained only with the secondary antibody was similar for the nonstimulated and the CD40L-stimulated DCs and is represented by the dotted line. Specific staining of nonstimulated DCs is represented by the open histogram, CD40L-stimulated DCs by the filled histogram. The results are representative of 4 independent experiments.
