Fig. 2.
Reduced cell yield after mo-DC development in the presence of Rapa.
Monocytes were cultured with IL-4 and GM-CSF in the absence (control) or presence of dexamethasone, CsA, FK506, or Rapa (10−9 M or 10−6 M as indicated). After a 6-day culture period with or without immunosuppressive drugs, cells were harvested and counted by trypan blue exclusion or by using the Coulter Counter. DMSO, in a concentration similar to the concentration used to test 10−6 M Rapa, served as a control. Cell survival in at least 4 independent experiments is expressed as mean (± SD) percentage of control DCs.
