American Society of Hematology

Fig. 3.

$Fig. 3. Rapa induces apoptosis in immature mo-DCs but not in monocytes. / (A) Freshly isolated monocytes were treated with 10−7 M Rapa. After 48 hours of incubation, cells were harvested and analyzed. The percentage of apoptosis was determined by using annexin V/PI staining. The results are representative of 5 independent experiments. (B-D) In vitro–generated mo-DCs were treated with 10−7 M Rapa. After 48 hours of incubation, cells were harvested and analyzed. The percentage of apoptosis was determined by using either annexin V/PI staining (B), Hoechst (C), or DNA staining to determine the sub-G0 fraction (D). The results are representative of 6 independent experiments.$

Rapa induces apoptosis in immature mo-DCs but not in monocytes.

(A) Freshly isolated monocytes were treated with 10⁻⁷ M Rapa. After 48 hours of incubation, cells were harvested and analyzed. The percentage of apoptosis was determined by using annexin V/PI staining. The results are representative of 5 independent experiments. (B-D) In vitro–generated mo-DCs were treated with 10⁻⁷ M Rapa. After 48 hours of incubation, cells were harvested and analyzed. The percentage of apoptosis was determined by using either annexin V/PI staining (B), Hoechst (C), or DNA staining to determine the sub-G₀ fraction (D). The results are representative of 6 independent experiments.

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