Rapa-induced apoptosis is regulated in a time- and dose-dependent manner.

Immature mo-DCs were cultured in RPMI–10% FCS supplemented with IL-4 and GM-CSF. Cells were incubated with increasing concentrations of Rapa or the vehicle for 24 or 48 hours. Apoptosis was detected by FACS analysis using annexin V–FITC and PI. The percentage of apoptosis demonstrates the percentage of annexin V⁺ PI⁻cells. Data shown are the mean ± SD of duplicate cultures. The results are representative of 2 independent experiments.